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KB-1® and KB-1® Plus bioaugmentation cultures enhance bioremediation success by introducing critical dechlorinating microbes to sites where they are absent or present at insufficient concentrations. Bioaugmentation leads to faster bioremediation, which means more efficient use of electron donors, reduced O&M requirements, lowering overall project costs.


SiREM's comprehensive quality control program and high quality shipping vessels ensure that each batch of bioaugmentation culture arrives at your site in prime condition ready to perform.

Our technical experts help you achieve bioaugmentation success with additional peace of mind provided by our bioaugmentation guarantee.

KB-1® the Gold Standard in Bioaugmentation

KB-1® is the most widely used bioaugmentation culture in the world having successfully introduced dechlorinating Dehalococcoides microbes at hundreds of sites across North America and Europe for the remediation of PCE, TCE, DCE, vinyl chloride and 1,2-DCA.

Questions Regarding Bioaugmentation and KB-1®

Q. What are signs that a site may require bioaugmentation with KB-1®?

A. There are several parameters that may indicate the need for KB-1® bioaugmentation:

  • Dechlorination stalls at cis-1,2-dichloroethene (cDCE) even after months of electron donor addition
  • Vinyl chloride (VC) accumulates with little or no ethene production
  • Gene-Trac® tests for total Dehalococcoides (Gene-Trac® Dhc) or vinyl chloride reductase (Gene-Trac® VC) are negative
  • Indigenous Dehalococcoides strains are present at low population densities or sparsely distributed based on Gene-Trac® testing

Q. What is the cost of Bioaugmentation?

A. Bioaugmentation with KB-1® or KB-1® Plus typically costs between $1 to $2 per cubic yard of saturated matrix depending on project scale and site conditions. Contact SiREM for a fixed price quotation for bioaugmentation.

Q. Do I need to bioaugment more than once?

A. No. Bioaugmentation is typically a one-time event. In rare cases where re-bioaugmentation is required our guarantee may provide extra culture free of charge.

Q. If Dehalococcoides are present at my site, should I still bioaugment with KB-1®?

A. Maybe. It depends on the need to achieve results quickly and the effectiveness of the indigenous Dehalococcoides (Dhc) community. A sparse or low population density of indigenous Dhc may require a long acclimation period which bioaugmentation can reduce. Also, research indicates that not all strains of Dhc have the same reductive dechlorination capacity; therefore, addition of more competent dechlorinators through bioaugmentation may be of benefit even if there is an existing population.

Q. Am I limited to which electron donors I can use with KB-1® or KB-1® Plus?

A. No. KB-1® and KB-1® Plus has been demonstrated to work with most commonly used electron donors, including alcohols (e.g., methanol, ethanol), organic acids (e.g., lactate), sugars (e.g., glucose, molasses) and slow release compounds such as emulsified vegetable oils. A general rule is any electron donor, that when fermented produces hydrogen, will support the activity of KB-1® and KB-1® Plus.

Q. Can bioaugmentation save money?

A. Yes. The introduction of a robust community of dechlorinating microorganisms reduces the use of electron donors on unwanted microbial processes such as sulfate reduction or methanogenesis. Also, rapid post-bioaugmentation dechlorination rates reduce site remediation time and associated O&M costs.

Q. How long will it take for bioaugmentation to have noticeable impacts at a site?

A. Impacts due to bioaugmentation, including complete dechlorination leading to increasing concentrations of ethene typically occur within 3 months of bioaugmentation and frequently faster. Under rare circumstances where bioaugmentation does not have a positive impact within 3 months SiREM's bioaugmentation guarantee covers rebioaugmentation culture costs.

Q. Is it difficult to obtain regulatory approval to use KB-1®?

A. No. KB-1® has been approved for use by regulatory authorities in more than half of US States, Canada, the United Kingdom, Denmark, Sweden, Germany, Hungary and Malaysia. As with other subsurface injectables, many jurisdictions require applicable permits. SiREM is pleased to provide manufacturing, quality control and safety information regarding our bioaugmentation products to support regulatory approvals.

For more information on KB-1®